pydna.user_cloning

Nicking & USER cloning

CGAuGTCGACTTAGATCTCACAGGCTTTTTTCAAGaCGGCCTTGAATTCAGTCATTTGGATCCGGCCGATC GCTACAGCTGAATCTAGAGTGTCCGAAAAAAGTTCTGCCGGAACTTAAGTCAGTAAACCTAGGCCGGCuAG

1. Digest both strands

2. Collect all linear ssDNA

3. Anneal all combinations

4. Keep ones present in original molecule

5. Rank by stability 6.