pydna.ladders

Agarose gel DNA ladders.

A DNA ladder is a list of FakeSeq objects that has to be initiated with Size (bp), amount of substance (mol) and Relative mobility (Rf).

Rf is a float value between 0.000 and 1.000. These are used together with the cubic spline interpolator in the gel module to calculate migartion distance from fragment length. The Rf values are calculated manually from a gel image. Exampel can be found in scripts/molecular_weight_standards.ods.